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Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue

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We typically recommend B12 therapy for individuals who have been diagnosed with B12 deficiency or who may be at risk due to factors such as age, certain medical conditions, or a vegan or vegetarian diet

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[DOI] [PMC free article] [PubMed] [Google Scholar] 11.Moncrieff J., Cooper R.E., Stockmann T., Amendola S., Hengartner M.P., Horowitz M.A