The concentration of dopamine was measured relative to standard solutions using Chromeleon 7.2 Chromatography Data System (Dionex, Thermo Scientific)
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2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)
Additionally, open field behavioral testing revealed that 6-9 month old mice in the control group were more active in the open field, however, as their age advanced these findings became insignificant [3]
