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Lipoyl adducts formed by changes in oxidative or catabolic stress can alter TCA cycle function to allow the cell to adapt to these insults, as demonstrated for transient lipoyl nitrosylation during macrophage activation 15,33

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10.1016/0016-5085(93)90638-S 112

After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)