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What is your reaction to protein maxxing, when you see someone eating six eggs and a plate of ground beef for breakfast

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Impaired protein metabolic functions cause the accumulation of ammonia and abnormal neurotransmitters, ultimately leading to hepatic encephalopathy

Genomic DNA was then isolated with the help of NucleoSpin Tissue XS from the clonal cell lines and subjected to sequencing to identify frameshift mutations by amplifying the genomic regions with the following primers: for ARL8A KO, forward 5- GATTCCGGGGCAGCGAGTCGT-3 and reverse 5-TACTAGGCCCCAAGCGCTCG-3, and for ARL8B KO, forward 5-TCATCTCCCGCCTGCTGGACT-3 and reverse 5-TCCAAATGCTGGCCCTCGG-3

Intensities of spots for blank and the housekeeping gene peptidylprolyl isomerase A were used for normalization of the data