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In the first step, glutamate cysteine ligase (GCL) catalyzes the ATP-dependent condensation of cysteine and glutamate to form the dipeptide gamma-glutamylcysteine (-GC) and then, in the second step, -glutamylcysteine reacts with glycine to form GSH catalyzed by GSH synthetase (GS)

Injectable protocols are not FDA-approved for cosmetic use, and long-term data from controlled trials is limited

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