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Relation of serum homocysteine and lipoprotein(a) concentrations to atherosclerotic disease in a prospective Finnish population based study

In normal pregnancies, biomarkers of oxidative stress, including malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), and thiobarbituric acid reactive substances (TBARS), are observed at elevated levels, reflecting a necessary augmentation in oxidative load to facilitate physiological processes [25]

Samples were centrifuged at 12,000 g for 10 min, and the supernatant was collected and measured for protein concentration using the BCA assay

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Cell processing and maintenance of cell cultures were described previously 7,9