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Mac Grory received grants from the National Institutes of Health and American Heart Association

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Reduced Shelf-Life: Because of the contamination risk, any peptide reconstituted with normal saline must be used immediately or stored for a very, very short period under refrigeration

The extracts were centrifuged at 10,000 rpm for 10 min at 4 C, and the supernatant was collected and used immediately for the assay at a dilution of 1:10 in PBS/EDTA

Gently swirl the vial in a circular motion until the powder dissolves completely into a clear, colorless liquid