glutathione column purification An alternative easy method for antibody and analysis of protein –protein interaction using GST fusion proteins immobilized onto glutathione–agarose optimized to yield up to 1mg of soluble GST tagged protein HPLC Method for Analysis off

122 Additionally, under glucose deprivation conditions, lactylation of nicotinamide nucleotide adenylyltransferase 1 (NMNAT1) in pancreatic adenocarcinoma cells maintains its enzymatic activity, accompanied by supporting the nuclear NAD + salvage pathway

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Quantitative PCR analysis of TNF-alpha and IL-1 beta mRNA levels in pediatric IBD mucosal biopsies

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Because traditional centrifugation-based methods are too slow to preserve ER metabolite content, we adapted an immunopurification strategy previously developed for other organelles 1315