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21, 2020 ( in press ) Mitchell, A,C., McHugh, M.A., Mack, T., Morand, M.M., Ngo, N.T., Kaur, P., Asea A

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Vranekovi J, Babi Bozovi I, Starcevi Cizmarevi N, et al

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To charge RHEB with GTPS (Abcam) or GDP (Sigma-Aldrich), RHEB was first diluted in buffer (50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM TCEP, 5 mM EDTA) and then supplemented with GDP or GTPS at a 30-fold molar excess