The eluent was prepared as follows: Eluent A was prepared by adding 15 mL of 500 mM 1-octanesulfonic acid sodium salt solution and 0.2 mL of trifluoroacetic acid to an appropriate volume of distilled water for HPLC (Nacalai Tesque), made up to a total of 1000 mL
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Although our knowledge of glucagon granule biogenesis and exocytosis is relatively rudimentary, this study, along with others, suggests that the mechanism of glucagon secretion shares considerable similarities with insulin secretion 19,36,37,38