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The membranes were then washed once with TTBS for 10 min and incubated in TTBS 1 with 0.5% skim milk supplemented with the following antibodies: a mouse monoclonal anti-human COX-1 (1:5000), a mouse monoclonal anti-human COX-2 (1:5000), a rabbit polyclonal anti-human mPGES-1 (1:2000

10.3390/nu11071544 105

Sulforaphane rescued rotenone-induced oxidative damage by activating the Nrf2 pathway ROS generation is a necessary by-product of oxidative metabolism and is tightly regulated in cells by various oxidant and antioxidant enzymes 39

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Furthermore, evidence indicates a direct association between levodopa sensitivity and the extent of -synuclein accumulation within the enteric nervous system (ENS) [46, 88]