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Cleaved protein was repurified from the cleaved fusion tag and TEV protease by loading on a Ni-NTA resin column and 5 ml of the flow through was applied to a size-exclusion column (HiLoad Sepharose 16/600 75 pg, GE Healthcare) on an KTA Pure system (GE Healthcare) at RT

They are also ideal for treating eyes exposed to air conditioning, central heating, pollution and hot or windy environments