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Twenty-four hours after transfection, cells were washed once in EDTA solution and dissociated from the flask in trypsin for 2 min at 37 C and seeded onto coated microscope slides (Hendley-Essex) in culture medium in a humidified incubator at 37 C with 5% CO 2

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Z series of at least 8 planes were obtained and maximum projection images were created and analysed

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