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GGT catalyses the breakdown of the -glutamyl bond in the substrate and generates p-nitroanilide (pNA), which is monitored kinetically by an increase in absorbance at 418 nm

For example, in the reaction with a hydroxide, we are comparing the leaving group ability of the hydroxide ion with the conjugate base of the amine: Comparing the p K a values of water (15.7 or 14 according to recent debates J

9, 2021 from Ankar, A

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Memarpoor-Yazdi M, Zare-Zardini H, Mogharrab N, Navapour L