Generally, protein purification entails five essential steps, i.e., extraction from biological matter, separation from lipids and nucleic acids (non-protein components), precipitation step, recovery of bulk protein from raw extract, utilization of hydrophobic or ion-exchange chromatography column for the separation of target protein from bulk protein, and more refined steps including affinity matrix to obtain highly refined target protein with high yields
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The copper component of the complex serves as an essential cofactor for lysyl oxidase, an enzyme that catalyzes the cross-linking of tropoelastin molecules into functional elastic fibers with the mechanical properties required for tissue resilience
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Conclusions apply only to what was actually tested