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The procedure involved extracting blood samples from filter paper using methanol, followed by drying the supernatant under nitrogen at 40 C, derivatization with butanolic-HCl, and reconstitution with 200 L of acetonitrile-water (50:50 v/v) containing 0.05 % formic acid to adjust pH to 4 for LC-MS/MS analysis

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FIGURE 1 Currently, N-acetylcysteine (NAC) is the only U.S

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