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Analyze gene ontology (GO) annotation with OmicsBean ( The Kyoto Encyclopedia of Genes and Genomes (KEGG) database was employed to analyze the canonical biochemical pathways

Master mix and Taqman gene expression assays for glyceraldehyde 3-phosphate dehydrogenase (GAPDH control) and target genes occludin (Hs05465837_g1) and claudin-1(Hs00221623_m1) were obtained from Applied Biosystems (Foster City, CA, USA)