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Following fixation in 4% paraformaldehyde (PFA), 5 dpf Brn3c:mGFP larvae, which express membrane-bound GFP in hair cells, were rinsed twice with phosphate-buffered saline (PBS) for 10 min each and then once with dH 2 o for 20 min

213 The DR nucleus is the largest and most significant conduit of forebrain serotonergic input

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While tirzepatide slows gastric emptying and reduces caloric intake through incretin receptor activation, 5-amino-1MQ works intracellularly to inhibit nicotinamide N-methyltransferase (NNMT), shifting cellular metabolism toward fat oxidation independent of appetite suppression