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Long-term use: Clinical trials only ran 12 weeks, so long-term safety data (6+ months continuous use) comes from anecdotal reports

The peptides short half-life of 4-6 hours means twice-daily dosing produces more consistent tissue levels than single daily administration

While this methylation reaction was historically considered a relatively minor metabolic pathway for nicotinamide clearance, research over the past decade has revealed that NNMT plays a surprisingly important and previously underappreciated role in cellular energy metabolism, NAD+ homeostasis, adipocyte biology, and whole-body metabolic regulation

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