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Protective effects of riboflavin and selenium on brain microsomal ca 2+-ATPase and oxidative damage caused by glyceryl trinitrate in a rat headache model

The reaction mixture contained 1 ml of sample and 2 ml of TBA-TCA-HCl reagent (0.37% thiobarbituric acid, 0.25 N hydrochloric acid and 15% trichloroacetic acid), placed in boiling water bath for 15 min, cooled at room temperature and centrifuged for 10 min

Therefore, all peptides inhibited the expression of TNF and pro-inflammatory IL-6 cytokine stimulated by LPS on terminally differentiated THP-1 cells

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This formulation is provided as a pre-standardised research solution to support consistent peptide composition, controlled laboratory handling, and reproducibility across non-clinical experimental workflows.