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RCAN1 depletion restores the mitochondrial network, together with oxygen consumption, indicating that the increased dosage of RCAN1 is responsible for the alteration of mitochondrial dynamics in the context of DS

BSO treatment was performed similarly as for ITCs by adding it (100 mM stock solution prepared in water) to the medium (either on its own or in combination with ITC) to obtain BSO concentrations ranging from 50 M to 700 M

The combination of methylcobalamin and folinic acid induced increases of 15% in total glutathione and 20% in reduced glutathione, respectively ( We then investigated the correlation between behavioral improvements and changes in intracellular glutathione concentrations

When appetite decreases, protein often drops unless the patient is intentional

Validation: The redesigned sensors were validated by: Comparing fluorescence intensity with the original sensor