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2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added

Data from a study showed that 4-HNE/protein adduct levels were higher in OA osteoblasts than in normal osteoblasts and when OA osteoblasts were treated with hydrogen peroxide [93]

Ozempic is part of a class of drugs called GLP-1 receptor agonists (GRa), which activate GLP-1 receptors

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No specific labeling by NbLumSyt1, fused to a GFP-tag, was observed after incubating neurons from Syt1 knockout (KO) mice 18 , confirming specificity as also further confirmed by immunoprecipitation experiments, in which endogenous Syt1 was enriched from wildtype (WT) but not from Syt1-KO brain extracts ( Fig