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Post incubation, the liberated IGF-1 was co-immunoaffinity extracted with the internal reference, using protein A/G MSIA pipette tips (from Thermo Fisher Scientific) loaded with antihuman IGF-1 antibody

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The disclosure also provides kits for preventing, treating or ameliorating the symptoms of a diseases or disorder in a mammal described herein

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FOXO4 is a member of the broader FOXO protein family, which has preserved both structural and functional characteristics across various animal species and has long been associated with pathways influencing aging