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NF-B (p65) DNA-binding activity analysis NF-B (p65) DNA-binding activity in the nuclei of homogenized liver tissues or cultured cells was measured using ELISA kits (Cayman, MI, USA) in accordance with the manufacturers instructions

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FOXO4-DRI also appears to influence insulin signaling, cell cycle regulation, and oxidative stress signaling pathways

doi: 10.1186/s12933-024-02178-2, 79 MagistrettiP

The supernatant containing the proteasomes were separated after centrifugation at 12,000 xg for 15 min at 4 C and were quantified and diluted to 1 g/L concentration with proteasome lysis buffer