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Side effects include: acne, blood clots, aggressiveness, hallucinations, mania, stomach pain, headaches, dizziness, high blood pressure, testicular or scrotal pain, premature male baldness, hear attack, stroke, and liver or kidney failure

BDNF ELISA on hippocampal homogenate, 24-72 h post-administration

or (3) treatment with erastin (10 M for 1224 h) to inhibit Cys 2 /glutamate antiporter (system x c )

IMPORTANT: MUST sanitize top part with rubbing alcohol before use

The cycle threshold (CT) values of gene expression were normalized to the housekeeping gene -actin, and the expression ratios were calculated using the CT method