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The quantification and normalization (DESeq method) and further downstream analyses of identification of differentially expressed genes (DEGs) were done by using R package

Furthermore, two-way ANOVA findings revealed a nonsignificant change in GLY levels in the midbrain and, thalamus and hypothalamus between the duration of IF and the diet regimen intervention ( F = 1.137, p = 0.338) and ( F = 3.046, p = 0.066), respectively

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These effects create a powerful metabolic shift toward fat utilization and energy expenditure