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Lipid peroxidation analysis Cells were harvested with TrypLE Select Enzyme (Thermo Fisher Scientific A1217702) and resuspended in 200 L PBS containing 5 M BODIPY 581/591 C11 (Invitrogen D3861) or 5 M MitoPerOx (Abcam ab146820) for 30 min to measure total lipid peroxidation or mitochondrial lipid peroxidation

But the good news is we have tested this treatment intravenously as well, and theres no sign of any toxicity, no sign of tumors at all after a year in mice, just blasting these genes but instead of three weeks which we do in the eye, blasting it for a year

Mller vom Hagen J., University of Tuebingen, Department of Neurology

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