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10.1016/j.cryobiol.2010.09.009 6

To reduce nonspecific binding, the sections were blocked for one hour at room temperature using a solution of 1% bovine serum albumin, 0.3% Triton X-100, and 10% goat serum in PBS, followed by three PBS washes

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A higher F2-Isoprostane/Creatinine Ratio Test result may indicate increased lipid peroxidation, meaning that fats in cell membranes or lipoproteins have undergone oxidative modification