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To further increase the physiological relevance and to adapt the model for drug testing, this study used expandable upcyte liver sinusoidal endothelial cells (LSECs) instead of HUVECs, which retain morphology and functionality observed in primary human LSECs 29

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Although preclinical studies have illustrated that pure nanotherapeutics possess some therapeutic functions, challenges still remain in their low accumulation at the targeted areas and easy leakage, which may cause therapeutic inefficiency to hinder subsequent clinical translation 23,24,25