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This process requires the presence of NAD+ as a cofactor

Transcriptional change in a cultured cell line is a hypothesis-generating observation

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Furthermore, we confirm that the initial glutathionylation of the glutaredoxin moiety and not the glutathione transfer to the roGFP2 moiety is rate-limiting for the oxidation of fusion constructs at physiological or low micromolar GSSG concentrations, thus allowing glutaredoxin structure-function analysis in vivo