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Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders (BenchMark, Invitrogen

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[DOI] [PubMed] [Google Scholar] 48.Nijland P.G., Witte M.E., van het Hof B., van der Pol S., Bauer J., Lassmann H., van der Valk P., de Vries H.E., van Horssen J

Spontaneous iron overload in Sprague-Dawley rats

in tumors, ROS stabilize HIF-1 to promote angiogenesis (VEGF) and immune checkpoint molecule expression (e.g., PD-L1), shaping an immunosuppressive microenvironment 104,105