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J Exp Med (2015) 212(9):134560

pJB-GST-HTS and the PCR fragment were prepared by BamHI and EcoRI digestion (Fw: AAAGGATCCATCATCATCATCATCATGGTCCGCTGGGCGTTCGTGGTATGGCTAGCAAAGGAGAAGAACTC, Rev: AAAGAATTCTCAGTTGTACAGTTCATCCATGCCATG)

The vitamin D 25-hydroxylase is a microsomal membrane localized enzyme encoded by the CYP2R1 (cytochrome P450 2R1) gene

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