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Preclinical studies explore how the peptide behaves in in vitro environments and animal models, examining factors such as cellular communication, inflammatory signaling, vascular-related pathways, and structural tissue responses

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Post-mortem brain tissue from AD patients and transgenic mouse models (e.g., APP/PS1, 5xFAD) consistently shows significant downregulation of glutathione peroxidase 4 protein expression, accompanied by characteristic ferroptotic morphological alterations such as mitochondrial shrinkage (189)