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E3 ligases are grouped into three classes according to their unique domains, including Really Interesting New Gene (RING) or U-box domain-containing E3 ligases, Homologous to E6AP C-terminus (HECT) domain-containing E3 ligases, and RING-between-RING (RBR) domain-containing E3 ligases (Morreale and Walden, 2016)

Regular Blood Testing: Regular monitoring of vitamin B12 levels through blood tests allows healthcare providers to assess if supplementation is necessary and adjust the dosage accordingly