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Cracked glass can introduce glass particles into the solution

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This is primarily done by analyzing the Amide I band (around 1600-1700 cm -1 originating from the C=O stretching and N-H in-phase bending vibration of the amide group), which is sensitive to protein secondary structure due to the variations in hydrogen bonding and dipole-dipole interactions within -helices, -sheets, and disordered regions (Sutherland, 1952