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The study was supported by the National Institutes of Health, the American Heart Association, the American Diabetes Association and the Juvenile Diabetes Research Foundation

First, the plasma samples were deproteinized with equal volume of the 5% 5-sulfosalicylic acid solution, vortexed vigorously for about 30 s, incubated for 10 min at 4 C, centrifuged at 10,000 g for 10 min at 4 C and supernatants were collected

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The N-terminus of GPCR is located extracellularly, whereas its C-terminus contains threonine and serine residues that promote the phosphorylation and intracellular signal transduction of downstream substrates via the G protein -subunit in vivo