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Genetically modified folate overproducing strains of some fungi and bacteria have been constructed, e.g., Ashbya gossypii , Escherichia coli , and Bacillus subtilis , the first being the best folate producer reported to date with folate titers of 6.6 mg/L (i.e., 146-fold higher than the wild strain) 631,632,633,634

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The authors of this study also found that supplementing zinc deficient animals with methyl donors during IVM restored histone H3K4 and increased IVF success rates (87)