coli BL21(DE3) containing plasmid pANT43 was inoculated from an overnight preculture at a 1:100 ratio in four 2 l baffled Erlenmeyer flasks filled with 400 ml TB medium containing 50 g ml 1 kanamycin sulfate and incubated at 37 C, 140 rpm using a 5 cm shaking diameter for 2.75 h
Botox accumulates in the nerve endings where it binds to vesicles or sacs containing a chemical called acetylcholine. This molecule has many actions in the body, including stimulating facial muscle contraction
A full list of survey questions can be found in Supplementary Table 24
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