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Subsequent studies have yielded similar success rates

When the OD600 reached 0.6, the cells induced with Isopropyl -d-1-thiogalactopyranoside (IPTG), and expressed at 22 C for 16 h

the animals were allowed to recover from surgery for several days before use in any experiment, and were fasted overnight prior to the the catheter was flushed with saline solution and a volume of saline equal to the volume of blood taken was injected (to maintain total blood volume)

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