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(3) Sequencing Paired-end sequencing (PE250) was performed on an Illumina NovaSeq 6000 platform (50,000 reads/sample)

The qPCR was executed on a qPCR instrument, and the relative gene expression data were processed using the 2 Ct method for analysis

doi: 10.3390/ijms20061305 76 Hurtado-NuezGECorts-RojoCSnchez-CejaSGMartnez-FloresHESalgado-GarcigliaRBartolom-CamachoMCet al

Evidence is lacking on the effects of the many dietary supplements on offer, such as vitamins, flavonoids, plant extracts, and trace elements

But this was somewhat overlooked in the study itself when they attempted to discuss their biological explanation for how red meat could harm the brain, mostly citing topics like saturated fat and TMAO that arent specific to processed red meat