B 12r and B 12s can be prepared from cyanocobalamin by controlled potential reduction, or chemical reduction using sodium borohydride in alkaline solution, zinc in acetic acid, or by the action of thiols
Not suitable for individuals with fish, shellfish, or other known allergies (adjust depending on your ingredient source)
The absorbance at 450 nm was measured via a microplate reader (Thermo Fisher, USA)
Rey F, Berardo C, Maghraby E, Mauri A, Messa L, Esposito L, Casili G, Ottolenghi S, Bonaventura E, Cuzzocrea S, Zuccotti G, Tonduti D, Esposito E, Paterniti I, Cereda C, Carelli S (2023) Redox imbalance in neurological disorders in adults and children
Yosefzon, Y., Soteriou, D., Feldman, A., Kostic, L., Koren, E., Brown, S., Ankawa, R., Sedov, E., Glaser, F., & Fuchs, Y