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40 On the other hand, high concentrations of NO favor the formation of reactive nitrogen and oxygen species, which are implicated in stress and subsequent physiological effects

Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

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As could be expected, the activity of core astrocyte-specific enzymes depend upon the formation of astrocytes, although some enzymes that later become astrocyte-specific are neuronal during early development