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Pena DA, Gasser B, Zanghellini J, Steiger MG, Mattanovich D (2018) Metabolic engineering of Pichia pastoris

Needle selection: Use 30-31 gauge insulin syringes with 0.3mL (30-unit) capacity

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Hydroxocobalamin is the standard UK preparation, administered intramuscularly for conditions like pernicious anaemia and gastrointestinal malabsorption B12 acts as a cofactor in DNA synthesis, red blood cell formation, and nervous system function, supporting energy metabolism pathways Common side effects include injection site reactions, mild gastrointestinal upset, and temporary urine discolouration, with serious reactions being rare NHS access requires GP assessment with blood tests

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