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2.2 Primary culture of myocytes and thermal stress trial 2.2.1 Primary culture of myocytes Juvenile fish, each weighing approximately 5 g, were dissected under sterile conditions to obtain muscle tissue, which was then cut into pieces and transferred to a 24-well cell culture plate

Literature suggests NACs impact on glutathione is limited, when GSH stores are within the physiological range, as feedback inhibition on glutamate-cysteine ligase may reduce GSH synthesis, 39 especially during development

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These biological and environmental discrepancies limit external validity and severely constrain the generalizability of preclinical findings to real-world clinical settings

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