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Total RNA was then isolated using the Total RNA Mini SI Isolation Spin-Kit (Applichem), and its concentration and purity were assessed with a Nanodrop 2000 spectrophotometer (Thermo Scientific)

Replacement of Glu170 in E170A/K resulted in a 5060% decrease of the k cat app values for GSH but had a rather minor effect on the k cat app (HEDS) values (Fig

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