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For instance, anabolism consumes energy in the form of ATP or NADH to synthesize biomolecules such as DNA and proteins, while catabolic reactions break down macromolecules, releasing energy in the form of ATP or NADH, which can be reutilized to generate NAD + 2

Dried metabolite extracts were resuspended in 100 L of LCMS-graded water following microcentrifugation for 5 minutes at 21,000 xg at 4 C to pellet any remaining insoluble debris

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