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While mild cases may improve with rest, chronic or recurrent bursitis often signals deeper issues such as tissue degeneration, poor biomechanics, or inflammation that the body can no longer resolve on its own

For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

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Goncalves, N

As noted above, this is expected and aligns with the protocols design