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Images were collected using a Leica TCS-SP2-AOBS confocal microscope

The resting membrane potential can be quantitatively described using the Goldman-Hodgkin-Katz (GHK) equation (Equation 1) (Shirai et al., 2017

Vomiting occurs less frequently than nausea but can be problematic when it does occur, particularly if it leads to dehydration or interferes with nutrition and medication absorption

In conclusion, our analysis provide insights into the dysbiosis of resident microbes and the molecular interplay between the microbiome and host, contributing to the understanding of gallstone formation