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Subsequently, the HUVECs are stained with a cell-permeable dye calcein AM (Corning, catalog number 354216) for 30 min, and then observed for capillary-like tubule formation using an inverted microscope

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Topical Skin Lightening Treatments: Topical treatments are generally suitable for mild to moderate pigmentation

The animal was removed from anesthesia and placed on a Marmarou foam block 31,32 and impacted midline (-2.5mm AP