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The program of qPCR reaction included 95 C for 10 min, 40 cycles of 95 C for 5 s and 60 C for 15 s

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Early Hum Dev 84(12):829838 Barateiro A, Miron VE, Santos SD, Relvas JB, Fernandes A, Ffrench-Constant C, Brites D (2013) Unconjugated bilirubin restricts oligodendrocyte differentiation and axonal myelination